CRISPR/Cas9 Technology
CRISPR/Cas9 is the most common gene editing technology currently which can be used to establish gene-edited mice including CKO/KO/KI/PM.
Advantages
1.High quality: Executing the most stringent quality control standards, Realtime PCR detection, 0 random insertion, 0 serial recombination
2.High efficiency: GPTech optimized efficient gene editing platform, higher success rate and shorter delivery cycle
3.Multi-modification: multiple loci or multiple genes of the target gene were knocked out at the same time, and the ultra-long target fragment 23Mbps was successfully KO
4.Multi-background: C57BL/6J, C57BL/6N, BALB/C, FVB, FVB, NOD/LTJ, NCG, etc.
CRISPR/Cas9 is a gene editing technology that achieves sprcific gene modification by cleavage of target gene loci by Cas9/sgRNA complexes. Compared with other technologies (TALEN, ZFN, ES technology), it is simple, fast, and low cost. Now, GPTech constructs more than 4000 mouse models per year through CRISPR/Cas9 technology.The construction capacity ranks among the best in the world. Details of making KO, CKO, KI, and PM gene-edited mice by CRISPR/Cas9 technology are as follows:
Gene knockout(Cas9 KO)
Conditional gene knockout（Cas9 CKO）
Gene knockin（Cas9-KI）
点突变（Cas9-PM）
