ES HR Technology
ES Homologous Recombination(HR) Technology for obtaining gene-edited mouse models is more suitable for establishing mouse models with complex editing strategies or larger inserts than CRISPR/Cas9 technology.
Advantages
1.Self-deletion of resistance genes: saving 3 months modeling time
2.BAC shooting technology: achieving 100-300kb large segment knock-in
3.Rich experience in model establishment: producing the first domestic CKO model in 2005; from then on, more than 700 ES target models have been successfully completed.
4.Multi-background: 129S6/SvEvTac, C57BL/6N
ES targeting technology is a gene editing technology that integrates foreign DNA into the genome of ES cells through homologous recombination, which obtains animal models through ES as the medium.The details of KO, CKO, ko-first and KI/PM gene-edited mice produced by ES Homologous Recombination Technology are as follows:
Gene Knockout（KO）
Knock out Strategy
Conditional gene knockout（CKO）
CKO Strategy
KO first
KO first Strategy
Gene knockin(KI)/Point mutation(PM）
Knock in Strategy
Time：2019-11-22