Facility Control and Maintenance
Mice intended for export are housed in our barrier facilities. Access to the facilities are strictly limited to trained laboratory and animal care personnel by key card access. Personal protective equipment (PPE) is required for entry into the facility which includes disposable gowns, shoe covers, gloves, hair bonnet and masks. Animal care personnel are dedicated to the facility and/or room. Animals are housed and maintained in positive-pressure, individually ventilated cages/units (IVC/U) which are changed every 1-2 weeks. Mice are provided with corncob bedding and paper nest enrichment and receive irradiated feed. All caging, bedding and enrichment are autoclaved prior to use. Processed water (reverse osmosis, chlorinated) is supplied by an Edstrom automatic watering system. Animals leaving the barrier cannot return to the facility.
The health status of all colonies is monitored quarterly by sampling from sentinel mice on a biweekly basis after exposure to soiled bedding. The pooled samples of soiled bedding are obtained from cages on their respective racks. The sentinel mice are 6-8 weeks of age and housed two per cage on each side of a rack unit, which contains 70/80 cages. Retired breeders are also tested as part of routine monitoring. The results are reported as positive tests/total test performed. Health monitoring includes necropsy, serologic testing, bacteriology, endo/ecto parasitology, histopathology, and DNA analysis. The tested items include all microbes included in the national microbial test standard of laboratory animals.
For imports, only specific pathogen free (SPF) animals are accepted and must be rederived and tested prior to release into the barrier. These animals are quarantined in isolators, and samples (including hair, feces or serology samples) of the received mice are submitted to GemPharmatech (GPT) microbial testing center. Animals can only be transported to the breeding animal rooms by cesarean section or embryo transfer once passing microbial tests.
|Ectromelia virus (Ect)||ELISA|
|Mouse hepatitis virus (MHV)||ELISA|
|Sendai virus (SV)||ELISA|
|Pneumonia virus of mice (PVM)||ELISA|
|Reovirus 3 (Reo-3)||ELISA|
|Minute virus of mice (MVM)||ELISA|
|Lymphocytic choriomeningitis (LCMV)||ELISA|
|Theiler's Mouse Encephalomyelitis Virus (TMEV)||ELISA|
|Mouse Adenovirus (FL) (MAD 1)||ELISA|
|Mouse Adenovirus (K87) (MAD 2)||ELISA|
|Polyoma Virus (POLY)||ELISA|
|Mouse Parvovirus (MPV)||ELISA|
|Mouse Cytomegalovirus (MCMV)||ELISA|
|Murine Norovirus (MNV)||ELISA|
|Epizootic Diarrhea of Infant Mice (EDIM)||ELISA|
|LDH elevating virus (LDV)||ELISA|
|Mouse Kidney Parvovirus (MKPV)||PCR|
BACTERIA & MYCOPLASMA
|Pathogenic dermal fungi||PCR|
|Escherichia coli O115 a,C,K (B)||Culture|
|Cilia-Associated Respiratory Bacillus (CARB)||ELISA|
PARASITES & PROTOZOA
|Ectoparasites, e.g., fleas, mites, and lice||Micro|
|Helminth, e.g., pinworms||Micro|
Additionally, GemPharmatech partners with several respected institutions and organizations to breed and house mice. For information on the health monitoring and testing practices of these facilities, please contact us.