To create a novel mouse model resource with greater genetic diversity, GemPharmatech used field-captured wild mice as the donor of genetic material and generated a series of new inbred strains through multi-generation cross breeding and genetic screening. In each one of such strains, only one chromosome is different from the parental inbred strain. Through primary phenotypic screening and systematic validation, we selected strains susceptible to metabolic diseases, such as obesity, diabetes, hyperlipidemia, and nonalcoholic steatohepatitis. These strains provide a new toolset for pathogenesis research and therapeutic evaluation.
Wild Mouse 750
Wild Mouse 750 (B6-Chr1YP1, No. D000750) has been developed to facilitate the study of obesity, fatty liver and other metabolic diseases. It possesses the wild mouse chromosome 1 on the C57BL/6J background. It exhibits metabolic sensitivity making it a better model for metabolic disease research.
- Increased genetic diversity through the introduction of an entire chromosome from a wild mouse.
- Clear genetic background (except Chr 1) and good reproductivity contributed by its parental C57BL/6J genome
- The genetic variations that contribute its metabolic phenotypes are naturally occurring, and located on chromosome 1.
After 8 weeks of age, the body weight of 750 mice increased gradually. ,At 26 weeks of age, 750 and B6J mice exhibited distinct tissue adiposity. The 750 mice had significantly enlarged adipose tissue at all depots. Mesenteric (m), subcutaneous (s), epididymal (e), white adipose tissue (WAT), brown adipose tissue (BAT).
After 8 weeks of age, the cholesterol levels of 750 mice increased gradually, and at 9 weeks of age, leptin and insulin levels were significantly higher than those in B6J control mice.
The disease characteristics of Wild Mouse 750 show more similarities to the human disease process than standard inbred mice and have a wide range of applications.
Obesity: This strain develops spontaneous obesity after 8 weeks of age, with significant increase in food intake and severe leptin resistance.
Abnormal glucose metabolism: Wild Mouse 750 exhibits mild and persistent hyperglycemia, insulin resistance, and glucose intolerance.
Abnormal lipid metabolism: Lipid levels (Chol, LDL-C, HDL-C) increase significantly after 8 weeks of age and continue to increase after Western diet (WD) feeding.
NAFLD/NASH: Aged mice exhibit a distinct fatty liver phenotype. WD feeding accelerates the development of NASH, including steatosis, lobular inflammation, and moderate fibrosis, with clinical NAS score of 7 and above.
Metabolic Diseases Analysis Platform
Not only Wild Mouse 750 is an ideal model for metabolic research as described above, it can also be used in preclinical efficacy evaluation of products targeting weight loss, hypoglycemia, hyperlipidemia, and NASH.
With our proprietary mouse models and experienced scientific team, GemPharmatech offers a one-stop solution for metabolic disease research, including phenotyping and drug efficacy evaluation services to analyze metabolic, behavioral, and hematological indicators.
|Metabolism||Neurobehavioral||Cardiovascular||Blood and Immunology||Bone|
|Metabolic cage||Water maze||Blood pressure||FACS, ELISA||Bone density|
|Physiological cage||Rotating bar||Electrocardiogram||CBC||Body composition|
|Glucose tolerance test||Open field||Cardiac ultrasound||Blood Biochemistry|